Composite

Part:BBa_K1300003:Design

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-16)


Constitutive T7 promoter + Lock (crRNA) + ccdB + dterm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 315


Design Notes

We had to ensure that this construct did not end up with two ribosomal binding sites. Therefore, we made sure to ligate the T7+cis with a coding ccdB (without the RBS). This would give us a construct with only one RBS.

Source

Built from parts that we had designed ourselves. 

T7+cis [1] & coding ccdB [2]

References